A Methodology for Implementing the Standardized Statistical Measures and Metrics for Public Services in Archival Repositories and Special Collections Libraries

The Rare Books and Manuscripts Section (RBMS) of the Association of College and Research Libraries (ACRL) and the Society for American Archivists (SAA) joint standard, Standardized Statistical Measures and Metrics for Public Services in Archival Repositories and Special Collections Libraries, provides a shared vocabulary and set of statistical measures that help archival and special collections repositories articulate their value as they share concrete evidence demonstrating the impact of their services. The standard is specifically designed to be system agnostic so that all repositories can use it. Many of the standard’s recommended measures, particularly those related to users and circulation, however, are challenging to implement without the use of a reading room management software system. This case study explores the adaptation of an existing quantitative assessment program to incorporate the measures and metrics suggested by the new RBMS/SAA standard without the use of specialized reading room management software

Analysis of magnetic nondestructive measurement methods for determination of the degradation of reactor pressure vessel steel

Nondestructive magnetic measurement methods can be successfully applied to determine the embrittlement of nuclear pressure vessel steel caused by neutron irradiation. It was found in previous works that reasonable correlation could be obtained between the nondestructively measured magnetic parameters and destructively determined ductile-to-brittle transition temperature. However, a large scatter of the measurement points was detected even in the cases of the non-irradiated reference samples. The reason for their scattering was attributed to the local inhomogeneity of material. This conclusion is verified in the present work by applying three different magnetic methods on two sets of Charpy samples made of two different reactor steel materials. It was found that by an optimal magnetic pre-selection of samples, a good, linear correlation can be found between magnetic parameters as well as the ductile-to-brittle transition temperature with low scattering of points. This result shows that neutron irradiation embrittlement depends very much on the local material properties

An annotated and illustrated checklist of Microgastrinae wasps (Hymenoptera, Braconidae) from the Canadian Arctic Archipelago and Greenland

The Microgastrinae (Hymenoptera: Braconidae) from ten islands of the Canadian Arctic Archipelago (CAA) and Greenland were studied based on 2,183 specimens deposited in collections. We report a total of 33 species in six genera, more than doubling the totals previously known. Most of the species (75.7%) have a distribution restricted to the Nearctic, with nine of those (27.3%) confirmed to be High Arctic endemics and another 10 species considered very likely to be High Arctic endemics as well – accounting for all of those, more than half of all species found are endemic to the region. The most diverse genera were Cotesia (10 species), Glyptapanteles (9 species), and Microplitis (7 species), representing 78.8% of the overall species diversity in the region. The six most frequently collected species comprised 84.7% of all examined specimens. The flight period for Microgastrinae in the High Arctic encompasses only two months, with activity peaking during the first half of July, when almost 40% of all available specimens were collected, and then plummeting in the first half to the end of August. Microgastrinae wasps from the High Arctic are currently known to parasitize eight species within four families of Lepidoptera: three species of Noctuidae, two each of Lymantridae and Nymphalidae, and one species of Pterophoridae. However, that information is very preliminary, as only six of the 33 species of microgastrines currently have associated host data. An annotated checklist, including photographs for 24 of the 33 species, is provided, as well as a key to all Microgastrinae genera present in the region

Teaching Orientation and Mobility Skills to Students with Autism and Vision Impairment in Public Schools: A Data-Based Study

Two students with autism, vision impairment, and intellectual disability participated in an orientation and mobility (O&M) intervention to travel in school settings using their folding canes. A multiple-baseline across participants design to determine the effectiveness of the intervention was used. The dependent variable was time taken to travel the specified route. The independent variable was O&M training. Results indicated that both participants took less time to travel during the intervention compared to the baseline. Students with vision impairment and autism can be trained using systematic O&M training. The O&M specialists working with children with autism and vision impairment should collect data and make data-based decisions while providing O&M instruction

Diversity, Prevalence, and Longitudinal Occurrence of Type II Toxin-Antitoxin Systems of Pseudomonas aeruginosa Infecting Cystic Fibrosis Lungs

Type II toxin-antitoxin (TA) systems are most commonly composed of two genes encoding a stable toxin, which harms the cell, and an unstable antitoxin that can inactivate it. TA systems were initially characterized as selfish elements, but have recently gained attention for regulating general stress responses responsible for pathogen virulence, formation of drug-tolerant persister cells and biofilms—all implicated in causing recalcitrant chronic infections. We use a bioinformatics approach to explore the distribution and evolution of type II TA loci of the opportunistic pathogen, Pseudomonas aeruginosa, across longitudinally sampled isolates from cystic fibrosis lungs. We identify their location in the genome, mutations, and gain/loss during infection to elucidate their function(s) in stabilizing selfish elements and pathogenesis. We found (1) 26 distinct TA systems, where all isolates harbor four in their core genome and a variable number of the remaining 22 on genomic islands; (2) limited mutations in core genome TA loci, suggesting they are not under negative selection; (3) no evidence for horizontal transmission of elements with TA systems between clone types within patients, despite their ability to mobilize; (4) no gain and limited loss of TA-bearing genomic islands, and of those elements partially lost, the remnant regions carry the TA systems supporting their role in genomic stabilization; (5) no significant correlation between frequency of TA systems and strain ability to establish as chronic infection, but those with a particular TA, are more successful in establishing a chronic infection

A Text-Messaging and Pedometer Program to Promote Physical Activity in People at High Risk of Type 2 Diabetes: The Development of the PROPELS Follow-On Support Program.

BACKGROUND: Mobile technologies for health (mHealth) represent a promising strategy for reducing type 2 diabetes (T2DM) risk. The PROPELS trial investigates whether structured group-based education alone or supplemented with a follow-on support program combining self-monitoring with pedometers and tailored text-messaging is effective in promoting and maintaining physical activity among people at high risk of T2DM. OBJECTIVE: This paper describes the iterative development of the PROPELS follow-on support program and presents evidence on its acceptability and feasibility. METHODS: We used a modified mHealth development framework with four phases: (1) conceptualization of the follow-on support program using theory and evidence, (2) formative research including focus groups (n=15, ages 39-79 years), (3) pre-testing focus groups using a think aloud protocol (n=20, ages 52-78 years), and (4) piloting (n=11). Analysis was informed by the constant comparative approach, with findings from each phase informing subsequent phases. RESULTS: The first three phases informed the structure, nature, and content of the follow-on support program, including the frequency of text messages, the need for tailored content and two-way interaction, the importance of motivational messages based on encouragement and reinforcement of affective benefits (eg, enjoyment) with minimal messages about weight and T2DM risk, and the need for appropriate language. The refined program is personalized and tailored to the individual's perceived confidence, previous activity levels, and physical activity goals. The pilot phase indicated that the program appeared to fit well with everyday routines and was easy to use by older adults. CONCLUSIONS: We developed a feasible and innovative text messaging and pedometer program based on evidence and behavior change theory and grounded in the experiences, views, and needs of people at high diabetes risk. A large scale trial is testing the effectiveness of this 4-year program over and above structured group education alone. TRIAL REGISTRATION: International Standard Randomized Controlled Trial Number (ISRCTN): 83465245; http://www.controlled-trials.com/ISRCTN83465245/83465245 (Archived by WebCite at http://www.webcitation.org/6dfSmrVAe)

Application of hierarchical clustering to identify high risk pests to Sitka spruce: Ireland as a case study

Invertebrate forest pests and pathogens can cause considerable economic losses and modern patterns of trade have facilitated the international movement of pest species on an unprecedented level. This upsurge in trade has increased the pathways available to high risk species, facilitating entry and potential establishment in nations where they were previously absent. To support policy and pest prioritization, pest risk analyses are conducted to decide ‘if’ and ‘how’ pests should be regulated in order to prevent entry or establishment; however, they cannot be carried out for every potential pest. This paper utilizes a hierarchical clustering (HC) approach to analyse distribution data for pests of Sitka spruce (Picea sitchensis (Bong.) Carr.) in order to identify species of high risk to Ireland, as well as potential source regions of these pests. The presence and absence of almost a 1000 pests across 386 regions globally are clustered based on their similarity of pest assemblages, to provide an objective examination of the highest risk pests to Irish forestry. Regional clusters were produced for each taxon analysed including the Coleoptera, Diptera, Hemiptera, Hymenoptera, Nematoda, Lepidoptera and the Fungi. The results produced by the HC analysis were interpreted with regard to biological realism and climate. Biologically meaningful clusters were produced for each of the groups, except for the Diptera and Nematoda, and each of the species analysed were ranked within their group by a quantitative risk index specific to the island of Ireland. The impact of uncertainty in the distribution data is also examined, in order to assess its influence over the final groupings produced. The outputs from this analysis suggest that the highest risk pests for Ireland’s Sitka spruce plantations will originate from within Europe. Ultimately, Ireland could benefit from seeking regulation for some of the higher ranking pests identified in this analysis. This analysis provides the first of its type for Sitka spruce, as well as its application in Ireland. It also serves to highlight the potential utility of HC as a ‘first approach’ to assessing the risk posed by alien species to hitherto novel regions

Medication burden in the first 5 years following diagnosis of type 2 diabetes: findings from the ADDITION-UK trial cohort.

INTRODUCTION: Individuals with screen-detected diabetes are likely to receive intensified pharmacotherapy to improve glycaemic control and general cardiometabolic health. Individuals are often asymptomatic, and little is known about the degree to which polypharmacy is present both before, and after diagnosis. We aimed to describe and characterize the pharmacotherapy burden of individuals with screen-detected diabetes at diagnosis, 1 and 5 years post-diagnosis. METHODS: The prescription histories of 1026 individuals with screen-detected diabetes enrolled in the ADDITION-UK trial of the promotion of intensive treatment were coded into general medication types at diagnosis, 1 and 5 years post-diagnosis. The association between change in the count of several medication types and age, baseline 10-year UK Prospective Diabetes Study (UKPDS) cardiovascular disease (CVD risk), sex, intensive treatment group and number of medications was explored. RESULTS: Just under half of individuals were on drugs unrelated to cardioprotection before diagnosis (42%), and this increased along with a rise in the number of prescribed diabetes-related and cardioprotective drugs. The medication profile over the first 5 years suggests multimorbidity and polypharmacy is present in individuals with screen-detected diabetes. Higher modeled CVD risk at baseline was associated with a greater increase in cardioprotective and diabetes-related medication, but not an increase in other medications. CONCLUSION: As recommended in national guidelines, our results suggest that treatment of diabetes was influenced by the underlying risk of CVD. While many individuals did not start glucose lowering and cardioprotective therapies in the first 5 years after diagnosis, more information is required to understand whether this represents unmet need, or patient-centered care. TRIAL REGISTRATION NUMBER: CNT00237549.This study was supported by the Welcome Trust (grant number G061895), the Medical Research Council (Grant numbers G0001164 and MC_UU_12015/4) and the National Institute for Health Research (Grant number RP-PG-0606-1259).This is the final version of the article. It first appeared from BMJ via http://dx.doi.org/10.1136/bmjdrc-2014-00007

Dynamic Nuclear Polarization-Enhanced Solid-State NMR Spectroscopy of GNNQQNY Nanocrystals and Amyloid Fibrils

Dynamic nuclear polarization (DNP) utilizes the inherently larger polarization of electrons to enhance the sensitivity of conventional solid-state NMR experiments at low temperature. Recent advances in instrumentation development and sample preparation have transformed this field and have opened up new opportunities for its application to biological systems. Here, we present DNP-enhanced [superscript 13]C–[superscript 13]C and [superscript 15]N–[superscript 13]C correlation experiments on GNNQQNY nanocrystals and amyloid fibrils acquired at 9.4 T and 100 K and demonstrate that DNP can be used to obtain assignments and site-specific structural information very efficiently. We investigate the influence of temperature on the resolution, molecular conformation, structural integrity and dynamics in these two systems. In addition, we assess the low-temperature performance of two commonly used solid-state NMR experiments, proton-driven spin diffusion (PDSD) and transferred echo double resonance (TEDOR), and discuss their potential as tools for measurement of structurally relevant distances at low temperature in combination with DNP.National Institutes of Health (U.S.) (Grant EB002804)National Institutes of Health (U.S.) (Grant EB003151)National Institutes of Health (U.S.) (Grant EB002026